Biochemical and Biophysical Research Communications 2006-01-06

Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals.

Junya Uwayama, Aki Hirayama, Toru Yanagawa, Eiji Warabi, Rika Sugimoto, Ken Itoh, Masayuki Yamamoto, Hiroshi Yoshida, Akio Koyama, Tetsuro Ishii

文献索引：Biochem. Biophys. Res. Commun. 339(1) , 226-31, (2006)

全文：HTML全文

摘要

Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I-/- mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I-/- than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I-/- mice. These results demonstrate that Prx I-/- mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.