Journal of Environmental Management 2010-11-01

Characterization of a fenpropathrin-degrading strain and construction of a genetically engineered microorganism for simultaneous degradation of methyl parathion and fenpropathrin.

Yuanfan Hong, Jin Zhou, Qing Hong, Qian Wang, Jiandong Jiang, Shunpeng Li

文献索引：J. Environ. Manage. 91(11) , 2295-300, (2010)

全文：HTML全文

摘要

A gram-negative fenpropathrin-degrading bacterial strain Sphingobium sp. JQL4-5 was isolated from the wastewater treatment sludge of an insecticide factory. Strain JQL4-5 showed the ability to degrade other pyrethroid insecticides, but it was not able to degrade methyl parathion. To enhance its degrading range of substrate, a methyl parathion hydrolase gene (mpd) was successfully introduced into the chromosome of strain JQL4-5 with a mini-Tn-transposon system. A genetically engineered microorganism (GEM) named JQL4-5-mpd resulted, which was capable of simultaneously degrading methyl parathion and fenpropathrin. Soil treatment results indicated that JQL4-5-mpd is a promising multifunctional bacterium in the bioremediation of multiple pesticide-contaminated environments.Copyright 2010 Elsevier Ltd. All rights reserved.