Acta Crystallographica Section F 2011-12-01

Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans.

Nobuhiro Suzuki, Young Min Kim, Zui Fujimoto, Mitsuru Momma, Hee Kwon Kang, Kazumi Funane, Masayuki Okuyama, Haruhide Mori, Atsuo Kimura

文献索引：Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 67(Pt 12) , 1542-4, (2011)

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摘要

Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 Å and belonged to space group P2(1), with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 Å, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 Å(3) Da(-1); assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 Å(3) Da(-1).