Biomaterials, Artificial Cells and Immobilization Biotechnology 1993-01-01

Studies on rifamycin oxidase immobilized on kappa-carrageenan gel.

U C Banerjee

文献索引：Biomater. Artif. Cells Immobilization Biotechnol. 21(5) , 665-74, (1993)

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摘要

Rifamycin oxidase from Curvularia lunata var. aeria was immobilized on kappa-carrageenan gel where the enzyme showed excellent catalyzing activity and operational stability. Factors affecting the activity of immobilized enzyme preparation such as pH and temperature were investigated. Thermostability of the immobilized enzyme preparation was checked at 30 and 40 degrees C and it was found that the thermostability of the immobilized rifamycin oxidase activity has increased compared to free enzyme. Transformation of rifamycin B to rifamycin S was also carried out with the immobilized enzyme preparation. Kappa-carrageenan immobilized rifamycin oxidase was also reused several times for the transformation of rifamycin B to rifamycin S.