A series of functionalized SBA-16 were synthesized and subsequently oxidized into sulfonic functionalized mesoporous silica mixing tetraethyl orthosilicate (TEOS) and 3-mercaptopropyltriethoxysilane (TMMPS), with triblock copolymers pluronic (F127) and cetyltrimethyl ammonium bromide (CTMABr) served as the mixing template by combination of hydrothermal and co-condensation method. The materials were characterized by X-ray diffraction, nitrogen gas sorption and SEM. The results show that the resulting functional materials belong to mesoporous materials when n(TEOS): n(TMMPS) is 3-8. The removal of Chrysoidine from water reaches the highest by functionalized mesoporous material when n(TEOS): n(TMMPS) is 7. Compared with different pH values, when the pH value ranges from 4-5 the adsorption reaches the highest.