Analytical Biochemistry 1984-04-01

Purification of quadrol for microsequencing in the spinning-cup sequenator.

G S Begg, B H Leslie, F J Morgan

文献索引：Anal. Biochem. 138(1) , 30-3, (1984)

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摘要

Treatment of Quadrol buffer with isothiocyanatophenylthiocarbamylaminoethylaminopropyl glass (DITC glass) substantially reduces the impurities observed when this buffer is used with the spinning cup sequenator. Use of DITC glass-treated Quadrol buffer permits identification of PTH-amino acids from protein degradations down to 100 pmol.