Voprosy Virusologii 1980-01-01

[Cultivation of herpesvirus in suspension culture on a microcarrier].

T A Bektemirov, F G Nagieva

文献索引：Vopr. Virusol. (5) , 615-8, (1980)

全文：HTML全文

摘要

The time course of RK-13 cell growth on a microcarrier DEAE-sephadex A-50 treated by different methods was studied. Treatment of the microcarrier according to the modified method was shown to provide the best conditions for cell attachment and growth. Herpes virus type I in monolayer cultures reached maximum titers at 2--3 days depending on the multiplicity of infection (6.5--7.83 lg CPD50). In cell cultures on the microcarrier at a high multiplicity of infection the virus titer reached maximum values (9.5 lg CPD50) as early as 24 hours postinfection.