Journal of chromatography. A 2015-11-13

Using the column wall itself as resistive heater for fast temperature gradients in liquid chromatography.

Ruben De Pauw, Matthias Pursch, Gert Desmet

文献索引：J. Chromatogr. A. 1420 , 129-34, (2015)

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摘要

A new system is proposed for applying fast temperature gradients in liquid chromatography. It consists of a 0.7 mm × 150 mm fused-silica column coated with a 50 μm Nickel-layer, which is connecting with a power source and a temperature control system to perform fast and reproducible temperature gradients using the column wall itself as a resistive heater. Applying a current of 4A and passive cooling results in a maximal heating and cooling rate of, respectively, 71 and -21 °C/min. Multi-segment temperature gradients were superimposed on mobile phase gradients to enhance the selectivity for three sets of mixtures (pharmaceutical compounds, a highly complex mixture and an insecticide sample). This resulted in a higher peak count or better selectivities for the various mixtures.Copyright © 2015 Elsevier B.V. All rights reserved.