The chemical composition of essential oil obtained by hydrodistillation from the dried fruits of Zosima absinthifolia was analysed by GC-MS. Twenty-four compounds were characterised in the oil. The major components of the oil were octyl acetate (87.48%), octyl octanoate (5.03%) and 1-octanol (2.37%). The oil showed modest to weak allelopatic effects and high antibacterial effects against Bacillus subtilis, Bacillus pumilus and further modest to strong effects on different bacteria and fungi.