To establish HPLC fingerprint of Bidens biternata from different habitats and determine the contents of hyperoside, isoquercetin, astragalin and bipinnatapolyacetylpside.Analysis was carried on Hypersil ODS C18 column (4.6 mm x 250 mm, 5.0 microm) with acetonitrile and 3% acetic acid as the mobile phase in a gradient elution. The contents of 4 components were determined simultaneously.The fingerprint of 10 populations were established and the data were analyzed by the similarity evaluation software. There were almost no differences between the similarities of 10 population, but the contents of 4 main compoerls were different among them.This method is stable and reliable which could be applied in quality assessment.
