Journal of Applied Genetics 2012-05-01

Genetic variation of Fusarium oxysporum isolates forming fumonisin B(1) and moniliformin.

Lidia Irzykowska, Jan Bocianowski, Agnieszka Waśkiewicz, Zbigniew Weber, Zbigniew Karolewski, Piotr Goliński, Marian Kostecki, Witold Irzykowski

文献索引：J. Appl. Genet. 53(2) , 237-47, (2012)

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摘要

Thirty single-spore isolates of a toxigenic fungus, Fusarium oxysporum, were isolated from asparagus spears and identified by species-specific polymerase chain reaction (PCR) and translation elongation factor 1-α (TEF) sequence analysis. In the examined sets of F. oxysporum isolates, the DNA sequences of mating type genes (MAT) were identified. The distribution of MAT idiomorph may suggest that MAT1-2 is a predominant mating type in the F. oxysporum population. F. oxysporum is mainly recognised as a producer of moniliformin-the highly toxic secondary metabolite. Moniliformin content was determined by high-performance liquid chromatography (HPLC) analysis in the range 0.05-1,007.47 μg g(-1) (mean 115.93 μg g(-1)) but, also, fumonisin B(1) was detected, in the concentration range 0.01-0.91 μg g(-1) (mean 0.19 μg g(-1)). There was no association between mating types and the mycotoxins biosynthesis level. Additionally, a significant intra-species genetic diversity was revealed and molecular markers associated with toxins biosynthesis were identified.