前往化源商城

Journal of Colloid and Interface Science 2011-04-01

Biotinylated thermoresponsive core cross-linked nanoparticles via RAFT polymerization and "click" chemistry.

Wenhui Lv, Li Liu, Yan Luo, Xiaojuan Wang, Yuwei Liu

文献索引:J. Colloid. Interface Sci. 356(1) , 16-23, (2011)

全文:HTML全文

摘要

A straightforward approach to the synthesis of "clickable" thermoresponsive core cross-linked (CCL) nanoparticles was developed. This approach was based on reversible addition-fragmentation chain transfer (RAFT) radical cross-linking polymerization of styrene and divinylbenzene with azide-functionalized poly(N-isopropylacrylamide) (PNIPAM-N(3)) as macro chain transfer agent in a selective solvent. Spherical nanoparticles with a diameter of 12nm were obtained after 24h polymerization. When the lyophilized CCL nanoparticles were dispersed in THF, spherical nanoparticles were observed, confirming the stability of CCL nanoparticles. The transmission electron microscopy (TEM) studies demonstrated that spherical nanoparticles and wormlike structure coexisted in the aqueous solution. The CCL nanoparticles have a lower critical solution temperature (LCST) at about 29.6°C, a little lower than that of PNIPAM homopolymer. Biotin molecules were conjugated to the surface of CCL nanoparticles via "click" chemistry in aqueous media. After bioconjugation, the LCST shifted to 28.3°C. The bioavailability of biotin to protein avidin was evaluated by a 4'-hydroxyazobenzene-2-carboxylic acid/avidin (HABA/avidin) binding assay and TEM.Copyright © 2011 Elsevier Inc. All rights reserved.

相关化合物

结构式 名称/CAS号 全部文献
二乙烯苯 结构式 二乙烯苯
CAS:1321-74-0
2-(对羟基苯偶氮)苯甲酸 结构式 2-(对羟基苯偶氮)苯甲酸
CAS:1634-82-8