Biochimica et Biophysica Acta 2006-02-01

Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy.

J Borejdo, J Talent, I Akopova, T P Burghardt

文献索引：Biochim. Biophys. Acta 1763(2) , 137-40, (2006)

全文：HTML全文

摘要

In order to measure the cycling of a few ( approximately 6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.