Guang pu xue yu guang pu fen xi = Guang pu 2002-12-01

[Determination of deoxyribonucleic acid with pararosaniline by a resonance light-scattering method].

Hai-yan Xiang, Xiao-ming Chen, Di-wu Zhou

文献索引：Guang Pu Xue Yu Guang Pu Fen Xi 22(6) , 1051-3, (2002)

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摘要

The method is based on the enhancement effect of the resonance light-scattering (RLS) of pararosaniline by DNA. In the pH rang of 0.5-1.5, the enhanced intensity of pararosaniline RLS at 355 nm is proportional to the concentration of DNA in the rang of 0.20-15 micrograms.mL-1. The detection limit is 36 ng.mL-1. This method is simple, rapid and has been applied satisfactorily to the determination of DNA in mixed samples.