Bioresource Technology 2013-03-01

Preparation and characterization of an extracellular polysaccharide produced by the deep-sea fungus Penicillium griseofulvum.

Yin Chen, Wenjun Mao, Baofeng Wang, Lina Zhou, Qianqun Gu, Yanli Chen, Chunqi Zhao, Na Li, Chunyan Wang, Jimiao Shan, Mengxia Yan, Cong Lin

文献索引：Bioresour. Technol. 132 , 178-81, (2013)

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摘要

The deep-sea fungus Penicillium griseofulvum produces an extracellular polysaccharide, Ps1-1, when grown in potato dextrose-agar medium. Ps1-1 was isolated from the fermented broth using ethanol precipitation, anion-exchange and size-exclusion chromatography. Ps1-1 is a galactomannan with a molecular weight of about 20 kDa, and a molar ratio of mannose and glucose of 1.1:1.0. On the basis of one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) and mass spectroscopic analyses, Ps1-1 is composed of a long chain of galactofuranan and a mannose core. The galactofuranan chain consists of (1→5)-linked β-galactofuranose, with additional branches at C-6 consisting of (1→)-linked β-galactofuranose residues and phosphate esters. The mannan core is composed of (1→6)-linked α-mannopyranose substituted at C-2 by (1→)-linked α-mannopyranose residues, disaccharide and trisaccharide units of (1→2)-linked α-mannopyranose. The investigation demonstrated that Ps1-1 was a galactofuranose-containing galactomannan differing from previously described extracellular polysaccharides.Copyright © 2012 Elsevier Ltd. All rights reserved.