China Journal of Chinese Materia Medica 2011-03-01

[Simultaneous determination of five glycosides in Aidi injection by RP-HPLC-ELSD].

Zhong Chen, Miaomiao Zhang, Xiaoran Li, Qiongming Xu, Shilin Yang

文献索引：Zhongguo Zhong Yao Za Zhi 36(6) , 706-8, (2011)

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摘要

To develop a RP-HPLC-ELSD method for the simultaneous determination of five glycosides in Aidi injection.The separation was carried out at 35 degrees C on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water as the mobile phases in gradient mode. The flow rate was 1.0 mL x min(-1). The SHIMADZU ELSD-LT II detector was used. The detector temperature was at 40 degrees C and the pressure of carrier gas N2 was 0.35 MPa.The linear response (the natural logarithm of peak areas with corresponding mass) ranges were 21.2-212 mg x L(-1) for syringin, 30.8-308 mg x L(-1) for ginsenoside Rg1, 24.8-248 mg x L(-1) for ginsenoside Re, 25.6-256 mg x L(-1) for ginsenoside Rb1, 31.2-312 mg x L(-1), for astragaloside IV, respectively. The average recoveries (n = 9) of five glycosides were greater than 97.6%, and RSD were less than 1.1%.The results demonstrated that this method had adequate accuracy and selectivity to measure the concentrations of five glycosides in Aidi injection.