Analytical Biochemistry 2008-02-15

DNA digestion to deoxyribonucleoside: a simplified one-step procedure.

Eoin P Quinlivan, Jesse F Gregory

文献索引：Anal. Biochem. 373(2) , 383-5, (2008)

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摘要

We present a simple and inexpensive 'one-step' protocol for the hydrolysis of DNA to deoxyribonucleosides. Unlike the older DNA hydrolysis protocol which is cumbersome and labor intensive, this new protocol is ideal for high-throughput assays and is suitable automation. Using this protocol we were able to hydrolyze several hundred samples within an 8-hour period. The new protocol is fully compatible with LC-MS/MS and gives similar recoveries for all five major deoxyribonucleosides when compared to the older protocol.