Journal of chromatography. B, Biomedical sciences and applications 2000-02-28

Preparation, evaluation and application of new pseudo-affinity chromatographic supports for penicillin acylase purification.

X Santarelli, V Fitton, N Verdoni, C Cassagne

文献索引：J. Chromatogr. B. Biomed. Sci. Appl. 739(1) , 63-72, (2000)

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摘要

New pseudo-affinity chromatographic supports for penicillin acylase were prepared and evaluated with three different samples: pure penicillin acylase, industrial clarified feedstock and crude extract. The different gels were studied for their purification fold (three to six) and their recovery power (80-100%). The best support was characterized by its dynamic capacity, (20 mg/ml) and its recovery power was tested at five flow-rates (30, 150, 300 and 750 cm/h) to determine the optimal flow-rate (300 cm/h). In addition we used cleaning in place to test the resistance to hard conditions of sanitization by 1 M NaOH (90% of recovery for 12 h of contact). These gels may therefore be used on an industrial scale.