Electrophoresis 2013-02-01

One-pot synthesis of (3-sulfopropyl methacrylate potassium)-silica hybrid monolith via thiol-ene click chemistry for CEC.

Huihui Yang, Yingzhuang Chen, Yixuan Liu, Lihua Nie, Shouzhuo Yao

文献索引：Electrophoresis 34(4) , 510-7, (2013)

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摘要

A novel (3-sulfopropyl methacrylate potassium)-silica hybrid monolithic column for CEC has been prepared by a simple one-pot approach based on efficient thiol-ene click chemistry. In this process, the polycondensation of hydrolyzed alkoxysilanes and in situ click reaction of vinyl groups on 3-sulfopropyl methacrylate potassium and thiol groups on the precondensed siloxanes simultaneously occurred in a pretreated capillary. Homogeneous monolithic matrix with large through-pores tightly bonded to the inner wall of the capillary was shown by optical microscope and SEM. The minimum plate height of this hybrid monolithic column was determined as 3.9 μm for thiourea. Anilines, alkylbenzenes, and phenols were well separated on this hybrid monolithic column by CEC, which indicated typical reversed-phase and cation-exchange chromatographic retention mechanisms of the column.© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.