Sen Zhao, Wei Ma, Liguang Xu, Xiaoling Wu, Hua Kuang, Libing Wang, Chuanlai Xu
文献索引：Biosens. Bioelectron. 68 , 593-7, (2015)
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For the first time, we demonstrated the fabrication of silver nanoparticle ornamented-gold nanoparticle pyramids (Ag-Au Pys) using an aptamer-based self-assembly process and investigated their surface-enhanced Raman scattering (SERS) properties in the detection of vascular endothelial growth factor (VEGF). Under optimized conditions, the SERS signal was negatively related to VEGF concentration over the range 0.01-1.0 fM and the limit of detection (LOD) was as low as 22.6 aM. The matrix effect and the specificity of this developed method were further examined, and the results showed that the superstructure sensor was ultrasensitive and highly selective. This developed aptamer-based SERS detection method suggests that it may be a promising strategy for a variety of sensing applications.Copyright © 2015 Elsevier B.V. All rights reserved.
