Analytical Biochemistry 2015-10-01

Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles.

Victoria Shpacovitch, Vladimir Temchura, Mikhail Matrosovich, Joachim Hamacher, Julia Skolnik, Pascal Libuschewski, Dominic Siedhoff, Frank Weichert, Peter Marwedel, Heinrich Müller, Klaus Überla, Roland Hergenröder, Alexander Zybin

文献索引：Anal. Biochem. 486 , 62-9, (2015)

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摘要

Recent proof-of-principle studies demonstrated the suitability of the surface plasmon resonance imaging (SPRi) technique for the detection of individual submicrometer and nanoparticles in solutions. In the current study, we used the SPRi technique for visualization of the binding of round-shaped viruses (inactivated influenza A virus) and virus-like particles (human immunodeficiency virus (HIV)-based virus-like particles) to the functionalized sensor surface. We show the applicability of the SPRi technique for the detection of individual virus-like particles in buffers without serum as well as in buffers containing different concentrations of serum. Furthermore, we prove the specificity of visualized binding events using two different pseudotypes of HIV virus-like particles. We also demonstrate the applicability of the SPRi technique for the determination of relative particle concentrations in solutions. Moreover, we suggest a technical approach, which allows enhancing the magnitude of binding signals. Our studies indicate that the SPRi technique represents an efficient research tool for quantification and characterization of biological submicrometer objects such as viruses or virus-like particles, for example. Copyright © 2015 Elsevier Inc. All rights reserved.