前往化源商城

Journal of Pharmaceutical Sciences 2014-12-01

Lysozyme-triggered epidermal growth factor release from bacterial cellulose membranes controlled by smart nanostructured films.

Guilherme Fadel Picheth, Maria Rita Sierakowski, Marco Aurelio Woehl, Lucy Ono, Axel Rulf Cofré, Luana Pasetti Vanin, Roberto Pontarolo, Rilton Alves De Freitas

文献索引:J. Pharm. Sci. 103(12) , 3958-65, (2014)

全文:HTML全文

摘要

A novel wound-dressing biodevice, sensitive to lysozyme, an enzyme commonly found at infected skin wounds, was assembled by the layer-by-layer deposition of nanopolymeric chitosan and alginate films onto oxidized bacterial cellulose membranes incorporated with epidermal growth factor (EGF). Distinct EGF release profiles were obtained according to specific stimuli caused by infection. In in vitro conditions simulating noninfected wounds, the EGF rate and burst release effect were reduced by three deposited layers (Mt /M∞ of 0.25 at 3 h) in a process dependent on the porosity of the compact chitosan-alginate complex. The importance of the organized structure was revealed when an infected wound was simulated by adding lysozyme to the release medium, thus inducing the formation of a loosely polyelectrolyte architecture that caused rapid EGF diffusion (Mt /M∞ of 0.75 at 30 min). The results indicate that the nanopolymeric layers were capable of slowly releasing EGF as required for normal wound repair and rapidly undergoing architectural transitions that allow the diffusion of massive amounts of drug to enhance the process of re-epithelialization. In summary, the proposed system comprises the roles of both wound dressing and local delivery mechanism to recognize infections and respond with a burst of EGF release.© 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

相关化合物

结构式 名称/CAS号 全部文献
盐酸 结构式 盐酸
CAS:7647-01-0
氢氧化钠 结构式 氢氧化钠
CAS:1310-73-2
溴化钠 结构式 溴化钠
CAS:7647-15-6
异硫氰酸荧光素酯 结构式 异硫氰酸荧光素酯
CAS:3326-32-7
异硫氰酸荧光素 结构式 异硫氰酸荧光素
CAS:27072-45-3
钾离子标准溶液 结构式 钾离子标准溶液
CAS:7440-09-7
3-乙基-2,4-戊烷二酮 结构式 3-乙基-2,4-戊烷二酮
CAS:1540-34-7
异硫氰酸罗丹明B 结构式 异硫氰酸罗丹明B
CAS:36877-69-7
冰醋酸 结构式 冰醋酸
CAS:64-19-7
次氯酸钠 结构式 次氯酸钠
CAS:7681-52-9