Journal of Immunology 2015-05-01

Survival of effector CD8+ T cells during influenza infection is dependent on autophagy.

Katrin Schlie, Ashley Westerback, Lindsay DeVorkin, Luke R Hughson, Jillian M Brandon, Sarah MacPherson, Izabelle Gadawski, Katelin N Townsend, Vincent I Poon, Mary A Elrick, Helene C F Côté, Ninan Abraham, E John Wherry, Noboru Mizushima, Julian J Lum

文献索引：J. Immunol. 194 , 4277-86, (2015)

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摘要

The activation and expansion of effector CD8(+) T cells are essential for controlling viral infections and tumor surveillance. During an immune response, T cells encounter extrinsic and intrinsic factors, including oxidative stress, nutrient availability, and inflammation, that can modulate their capacity to activate, proliferate, and survive. The dependency of T cells on autophagy for in vitro and in vivo activation, expansion, and memory remains unclear. Moreover, the specific signals and mechanisms that activate autophagy in T effector cells and their survival are not known. In this study, we generated a novel inducible autophagy knockout mouse to study T cell effector responses during the course of a virus infection. In response to influenza infection, Atg5(-/-) CD8(+) T cells had a decreased capacity to reach the peak effector response and were unable to maintain cell viability during the effector phase. As a consequence of Atg5 deletion and the impairment in effector-to-memory cell survival, mice fail to mount a memory response following a secondary challenge. We found that Atg5(-/-) effector CD8(+) T cells upregulated p53, a transcriptional state that was concomitant with widespread hypoxia in lymphoid tissues of infected mice. The onset of p53 activation was concurrent with higher levels of reactive oxygen species (ROS) that resulted in ROS-dependent apoptotic cell death, a fate that could be rescued by treating with the ROS scavenger N-acetylcysteine. Collectively, these results demonstrate that effector CD8(+) T cells require autophagy to suppress cell death and maintain survival in response to a viral infection. Copyright © 2015 by The American Association of Immunologists, Inc.