Bioresource Technology 2015-01-01

Improved ethanol tolerance and ethanol production from glycerol in a streptomycin-resistant Klebsiella variicola mutant obtained by ribosome engineering.

Toshihiro Suzuki, Kohei Seta, Chiaki Nishikawa, Eri Hara, Toshiya Shigeno, Toshiaki Nakajima-Kambe

文献索引:Bioresour. Technol. 176 , 156-62, (2014)



To improve the ethanol tolerance of the Klebsiella variicola strain TB-83, we obtained the streptomycin-resistant, ethanol-tolerant mutant strain TB-83D by a ribosome engineering approach. Strain TB-83D was able to grow in the presence of 7% (v/v) ethanol and it showed higher ethanol production than strain TB-83. Examination of various culture conditions revealed that yeast extract was essential for ethanol production and bacterial growth. In addition, ethanol production was elevated to 32g/L by the addition of yeast extract; however, ethanol production was inhibited by formate accumulation. With regard to cost reduction, the use of corn steep liquor (CSL) markedly decreased the formate concentration, and 34g/L ethanol was produced by combining yeast extract with CSL. Our study is the first to improve ethanol tolerance and productivity by a ribosome engineering approach, and we found that strain TB-83D is effective for ethanol production from glycerol.Copyright © 2014 Elsevier Ltd. All rights reserved.


结构式 名称/CAS号 全部文献
甘油 结构式 甘油
乙醇 结构式 乙醇
磷酸二氢钾 结构式 磷酸二氢钾
氯化铵 结构式 氯化铵
6-苄氨基嘌呤 结构式 6-苄氨基嘌呤