Chemoenzymatic synthesis of optically active alcohol and β-amino-acid derivative containing the difluoromethylene group

T Ema, T Kadoya, K Akihara, T Sakai

文献索引：Ema, Tadashi; Kadoya, Taro; Akihara, Kumiko; Sakai, Takashi Journal of Molecular Catalysis B: Enzymatic, 2010 , vol. 66, # 1-2 p. 198 - 202

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被引用次数: 8

摘要

The bioreduction of α, α-difluorinated ketones, ethyl 2, 2-difluoro-3-oxobutanoate (2a) and 2, 2-difluoro-1-phenyl-1, 3-butanedione (2b), with cells of recombinant Escherichia coli overproducing SCR (Saccharomyces cerevisiae carbonyl reductase from bakers' yeast) and GDH (glucose dehydrogenase from Bacillus megaterium) gave enantiomerically pure alcohols, ethyl (S)-2, 2-difluoro-3-hydroxybutanoate ((S)-1a) and (S)-2, 2-difluoro-3- ...