LC–MS and NMR characterization of the purple chromophore formed in the o-aminobenzaldehyde assay of dihydrodipicolinate synthase

V Mitsakos, SRA Devenish, PA O'Donnell…

文献索引：Mitsakos, Voula; Devenish, Sean R.A.; O'Donnell, Paul A.; Gerrard, Juliet A.; Hutton, Craig A. Bioorganic and Medicinal Chemistry, 2011 , vol. 19, # 4 p. 1535 - 1540

全文：HTML全文

被引用次数: 6

摘要

The enzyme dihydrodipicolinate synthase (DHDPS) has been widely investigated as a target for new antibiotics. The o-aminobenzaldehyde (o-ABA) assay is routinely used as a highly specific, if qualitative, tool for DHDPS purification, whereby fractions containing active DHDPS appear purple upon addition of o-ABA. The purple adduct absorbs in the visible region (540nm) but has never been characterized in the 50years since it was first reported ...