A light-activated probe of intracellular protein kinase activity
WF Veldhuyzen, Q Nguyen, G McMaster…
文献索引：Veldhuyzen, Willem F.; Nguyen, Quan; McMaster, Gary; Lawrence, David S. Journal of the American Chemical Society, 2003 , vol. 125, # 44 p. 13358 - 13359
The first example of a photoactivated probe of intracellular enzymatic activity is described. The caged derivative of a fluorescent protein kinase C peptide-based sensor was prepared by modifying the free hydroxyl group of a phosphorylatable serine moiety with a photolabile appendage that blocks phosphoryl transfer. We have demonstrated that the caged sensor allows one to (1) sample PKC activity with exquisite temporal precision,(2) control the ...
