Analytical biochemistry

Relative quantitation of glycans using stable isotopic labels 1-(d0/d5) phenyl-3-methyl-5-pyrazolone by mass spectrometry

P Zhang, Y Zhang, X Xue, C Wang, Z Wang…

文献索引：Zhang, Ping; Zhang, Ying; Xue, Xiangdong; Wang, Chenjian; Wang, Zhongfu; Huang, Linjuan Analytical Biochemistry, 2011 , vol. 418, # 1 p. 1 - 9

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被引用次数: 27

摘要

A deuterium reagent, 1-(d5) phenyl-3-methyl-5-pyrazolone (d5-PMP), has been synthesized and used for relative quantitative analysis of oligosaccharides by mass spectrometry (MS) using d0/d5-PMP stable isotopic labeling. Previously reported permethylation-based isotopic labels generate variable mass differences, and reductive amination-based isotopic labels cause a loss of some acid-labile groups in carbohydrates. In contrast, d0/d5-PMP ...