Abstract This study presents the synthesis and spectral analysis of new derivatives of 1, 2, 4- triazole-3-thione and 1, 3, 4-thiadiazole. New compounds were prepared by cyclization reaction of acyl thiosemicarbazide derivatives in the presence of alkaline and acidic media. All synthesized compounds were screened for their in vitro antibacterial activity by using the agar dilution technique. Six of the compounds had potential activity against Gram-positive ...