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Fingerprint lipolytic enzymes with chromogenic p-nitrophenyl esters of structurally diverse carboxylic acids

L Qian, JY Liu, JY Liu, HL Yu, CX Li, JH Xu

文献索引:Qian, Le; Liu, Jia-Yan; Liu, Jia-Ying; Yu, Hui-Lei; Li, Chun-Xiu; Xu, Jian-He Journal of Molecular Catalysis B: Enzymatic, 2011 , vol. 73, # 1-4 p. 22 - 26

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被引用次数: 11

摘要

A series of structurally diverse chromogenic esters, including a new compound (4- nitrophenyl 2-methylpentanoate), has been synthesized, constituting an array of 17 substrates which could be applied to rapidly fingerprint the activity of lipases or esterases to reveal their substrates specificity and functional characteristics. Combined with genetic technology such as “data mining” and directed evolution, such fingerprints might be a ...

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