xenob io t ic a , 1998, vol . ... BrieŊy, the incubation mixture (Ūnal volume 25 l l) for the PCR reaction consisted of 10x Ex Taq buåer, 4 dNTPs, primers, genomic DNA as a template (25 ng) and 0ą 5 U Ex Taq polymerase (Takara, Kyoto, Japan). ... Biochemistry, 28, 4993ą4999. ...