Efficient ribosomal peptidyl transfer critically relies on the presence of the ribose 2'-OH at A2451 of 23S rRNA

…, B Wotzel, R Rieder, R Micura, N Polacek

文献索引：Erlacher, Matthias D.; Lang, Kathrin; Wotzel, Brigitte; Rieder, Renate; Micura, Ronald; Polacek, Norbert Journal of the American Chemical Society, 2006 ,  vol. 128,  # 13  p. 4453 - 4459

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被引用次数: 65

摘要

The ribosomal peptidyl transferase center is a ribozyme catalyzing peptide bond synthesis in all organisms. We applied a novel modified nucleoside interference approach to identify functional groups at 9 universally conserved active site residues. Owing to their immediate proximity to the chemical center, the 23S rRNA nucleosides A2451, U2506 and U2585 were of particular interest. Our study ruled out U2506 and U2585 as contributors of vital ...