Analytical chemistry

Detection of alkaline phosphatase using surface-enhanced Raman spectroscopy

C Ruan, W Wang, B Gu

文献索引：Ruan, Chuanmin; Wang, Wei; Gu, Baohua Analytical Chemistry, 2006 , vol. 78, # 10 p. 3379 - 3384

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被引用次数: 105

摘要

A new approach was developed to detect the activity of alkaline phosphatase (ALP) enzyme at ultralow concentrations using a surface-enhanced Raman scattering (SERS) technique. The approach is based on the use of gold nanoparticles as a SERS material whereas 5- bromo-4-chloro-3-indolyl phosphate (BCIP) is used as a substrate of ALP. The enzymatic hydrolysis of BCIP led to the formation of indigo dye derivatives, which were found to be ...