Synthesis of fluorescence-labeled sphingosine and sphingosine 1-phosphate; effective tools for sphingosine and sphingosine 1-phosphate behavior

T Hakogi, T Shigenari, S Katsumura, T Sano…

文献索引：Hakogi, Toshikazu; Shigenari, Toshihiko; Katsumura, Shigeo; Sano, Takamitsu; Kohno, Takayuki; Igarashi, Yasuyuki Bioorganic and Medicinal Chemistry Letters, 2003 , vol. 13, # 4 p. 661 - 664

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被引用次数: 27

摘要

A fluorescence-labeled sphingosine and sphingosine 1-phosphate have been successfully synthesized from the oxazolidinone methyl ester derived from glycidol via monoalkylation and the stereoselective reduction of the resulting ketone. The labeled sphingosine was converted into its phosphate by treatment with sphingosine kinase 1 (SPHK1) from mouse, and in platelets, and it was incorporated into the Chinese Hamster Ovarian (CHO) cells. In ...