Use of L-lysine fluorescence derivatives as tracers to enhance the performance of polarization fluoroimmunoassays. A study using two herbicides as model antigens
GI Hatzidakis, AM Tsatsakis, EK Krambovitis…
文献索引：Hatzidakis, George I.; Tsatsakis, Aristidis M.; Krambovitis, Elias K.; Spyros, Apostolos; Eremin, Sergei A. Analytical Chemistry, 2002 , vol. 74, # 11 p. 2513 - 2521
Fluorescence polarization immunoassay (FPIA) is a convenient homogeneous assay, the use of which is restricted in environmental analysis by low sensitivity and matrix effects. We selected the herbicides 2, 4D and 2, 4, 5T to synthesize new l-lysine-based fluorescent tracers using solid-phase chemistry. In addition, three different immunogens of 2, 4, 5T were prepared for immunization and antibody production. The new tracers and antibodies were ...
