Chemistry & biology

An artificial pathway to 3, 4-dihydroxybenzoic acid allows generation of new aminocoumarin antibiotic recognized by catechol transporters of E. coli

S Alt, N Burkard, A Kulik, S Grond, L Heide

文献索引：Alt, Silke; Burkard, Nadja; Kulik, Andreas; Grond, Stephanie; Heide, Lutz Chemistry and Biology, 2011 , vol. 18, # 3 p. 304 - 313

全文：HTML全文

被引用次数: 17

摘要

An artificial operon was synthesized, consisting of the genes for chorismate pyruvate-lyase of E. coli and for 4-hydroxybenzoate 3-hydroxylase of Corynebacterium cyclohexanicum. This operon, directing the biosynthesis of 3, 4-dihdroxybenzoate, was expressed in the heterologous expression host Streptomyces coelicolor M512, together with a modified biosynthetic gene cluster for the aminocoumarin antibiotic clorobiocin. The resulting strain ...