Substrate specificity of the bovine serum amine oxidase and in situ characterisation of aminoaldehydes by NMR spectroscopy

…, R Søndergaard, T Friis, U Anthoni, PH Nielsen…

文献索引：Houen, Gunnar; Struve, Casper; Sondergaard, Roar; Friis, Tina; Anthoni, Uffe; Nielsen, Per H.; Christophersen, Carsten; Petersen, Bent O.; Duus, Jens . Bioorganic and Medicinal Chemistry, 2005 , vol. 13, # 11 p. 3783 - 3796

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被引用次数: 7

摘要

The oxidation of spermidine or homospermidine with bovine serum amine oxidase (BSAO) was monitored in situ, using proton nuclear magnetic resonance spectroscopy in water with 10% D2O. NMR assignments were performed by spin decoupling and COSY spectra or by comparison with data from synthetic aminoaldehydes. The results represent the first in situ characterisation of the highly reactive aminoaldehydes and showed oxidation at the N1 ...