Tetrahedron

Photoaffinity labeling of bovine rhodopsin

R Sen, AK Singh, V Balogh-Nair, K Nakanishi

文献索引：Sen, Ranjan; Singh, Anil K.; Balogh-Nair, Valeria; Nakanishi, Koji Tetrahedron, 1984 , vol. 40, # 3 p. 493 - 500

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被引用次数: 14

摘要

Photoaffinity labeled (3-diazoacetoxy)-9-cis-retinal (1) and (9-methylenediazoacetoxy)-9-cis- retinal (20) were synthesized and bound to absorption maxima at 465 and 460 nm respectively. Binding studies established that synthetic retinals 1 and 2 bind to the natural binding site and that the integrity of the diazoacetoxy photoaffinity label is preserved in the process. Incorporation of 3-(O14COCHN2)-labeled 9-cis retinal could be conveniently ...