Heterologous Over??expression of α??1, 6??Fucosyltransferase from Rhizobium sp.: Application to the Synthesis of the Trisaccharide β??D??GlcNAc (1→ 4)??[α??L??Fuc??(1→ …
A Bastida, A Fernández??Mayoralas…
文献索引：Bastida, Agatha; Fernandez-Mayoralas, Alfonso; Gomez Arrayas, Ramon; Iradier, Fatima; Carretero, Juan Carlos; Garcia-Junceda, Eduardo Chemistry - A European Journal, 2001 , vol. 7, # 11 p. 2390 - 2397
Abstract An efficient heterologous expression system for overproduction of the enzyme α-1, 6-Fucosyltransferase (α-1, 6-FucT) from Rhizobium sp. has been developed. The gene codifying for the α-1, 6-FucT was amplified by PCR using specific primers. After purification, the gene was cloned in the plasmid pKK223-3. The resulting plasmid, pKK1, 6FucT, was transformed into the E. coli strain XL1-Blue MRF′. The protein was expressed both as ...
