On the use of nonfluorescent dye labeled ligands in FRET-based receptor binding studies

C Tahtaoui, F Guillier, P Klotz, JL Galzi…

文献索引：Tahtaoui, Chouaib; Guillier, Fabrice; Klotz, Philippe; Galzi, Jean-Luc; Hibert, Marcel; Ilien, Brigitte Journal of Medicinal Chemistry, 2005 , vol. 48, # 24 p. 7847 - 7859

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被引用次数: 27

摘要

The efficiency of fluorescence resonance energy transfer (FRET) is dependent upon donor- acceptor proximity and spectral overlap, whether the acceptor partner is fluorescent or not. We report here on the design, synthesis, and characterization of two novel pirenzepine derivatives that were coupled to patent blue VF and pinacyanol dyes. These nonfluorescent compounds, when added to cells stably expressing enhanced green fluorescent protein ( ...