Synthesis and stability of novel terminal phosphate-labeled nucleotides

B Reynolds, R Miller, J Williams…

文献索引：Reynolds, Bambi; Miller, Rachel; Williams, John G.; Anderson, Jon P. Nucleosides, Nucleotides and Nucleic Acids, 2008 , vol. 27, # 1 p. 18 - 30

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被引用次数: 3

摘要

Novel compounds consisting of a nucleotide triphosphate labeled with a PEG linker and various terminal groups attached to the γ-phosphate of the nucleotide were constructed for use in efforts to produce a new class of DNA sequencer. The stability of these novel compounds was investigated to determine their utility as sequencing reagents. Hydrolysis rate constants were measured for both the natural nucleoside triphosphate dATP and ...