A high throughput screening strategy for the assessment of nitrile-hydrolyzing activity towards the production of enantiopure β-hydroxy acids

TM Coady, LV Coffey, OR Catherine, EB Owens…

文献索引：Coady, Tracey M.; Coffey, Lee V.; O'Reilly, Catherine; Owens, Erica B.; Lennon, Claire M. Journal of Molecular Catalysis B: Enzymatic, 2013 , vol. 97, p. 150 - 155

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被引用次数: 6

摘要

Abstract Nitrile hydrolysing enzymes have found wide use in the pharmaceutical industry for the production of fine chemicals. This work presents a strategy that facilitates the rapid identification of bacterial isolates demonstrating nitrile hydrolysing activity. The strategy incorporates toxicity, starvation and induction studies along with subsequent colorimetric screening for activity, further focusing the assessment towards the substrates of interest. ...