Tetrahedron: Asymmetry

Expanded structural and stereospecificity in peptide synthesis with chemically modified mutants of subtilisin

K Khumtaveeporn, G DeSantis, JB Jones

文献索引：Khumtaveeporn, Kanjai; DeSantis, Grace; Jones, J. Bryan Tetrahedron Asymmetry, 1999 , vol. 10, # 13 p. 2563 - 2572

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被引用次数: 23

摘要

Employing the strategy of combined site directed mutagenesis and chemical modification, we previously generated chemically modified mutant enzymes (CMMs) of subtilisin Bacillus lentus (SBL). We now report the use of these SBL-CMMs for peptide coupling reactions. The SBL-CMMs exhibit dramatically altered substrate specificity, including the acceptance of d- amino acid acyl donors, generating dipeptides containing d-Phe, d-Ala and d-Glu in up to ...