A mechanism for the addition of multiple moles of glutamate by folylpolyglutamate synthetase

…, PD Colman, RA Forsch, A Rosowsky

文献索引：Moran; Colman; Forsch; Rosowsky Journal of Medicinal Chemistry, 1984 , vol. 27, # 10 p. 1263 - 1267

被引用次数: 18

摘要

The role of the a-carboxyl group in methotrexate (MeAPA-Glu) and the y-glutamate derivative of methotrexate (MeAPA-Glu-Glu) in the reaction catalyzed by folylpolyglutamate synthetase (FPGS) has been investigated. MeAPA-Glu and MeAPA-Glu-Glu were accepted as substrates by the same FPGS species contained in an (NH4) pS04 precipitate of mouse liver protein, as judged by a lack of additivity of product formation at saturating ...