Phytochemistry

Pinus taeda phenylpropenal double-bond reductase: purification, cDNA cloning, heterologous expression in Escherichia coli, and subcellular localization in P. taeda

H Kasahara, Y Jiao, DL Bedgar, SJ Kim, AM Patten…

文献索引：Kasahara, Hiroyuki; Jiao, Ying; Bedgar, Diana L.; Kim, Sung-Jin; Patten, Ann M.; Xia, Zhi-Qiang; Davin, Laurence B.; Lewis, Norman G. Phytochemistry, 2006 , vol. 67, # 16 p. 1765 - 1780

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被引用次数: 33

摘要

A phenylpropenal double-bond reductase (PPDBR) was obtained from cell suspension cultures of loblolly pine (Pinus taeda L.). Following trypsin digestion and amino acid sequencing, the cDNA encoding this protein was subsequently cloned, with the functional recombinant protein expressed in Escherichia coli and characterized. PPDBR readily converted both dehydrodiconiferyl and coniferyl aldehydes into dihydrodehydrodiconiferyl ...