Abstract A second-generation sulfonyl azide-based fluorescent probe, 2, 6-DNS-Az, has been developed for the quantitative detection of H 2 S in aqueous media such as phosphate buffer and bovine serum. Compare to the first-generation 1, 5-DNS-Az probe, this probe shows both high sensitivity in phosphate buffer without the need for addition of surfactant and selectivity for sulfide over other anions and biomolecules, and thus can be used as a ...