... DHA-binding experiments by incubating 300 μl of the membrane preparation with six to eight increasing concentrations of [ 3 H]DHA (1.1 to 11.0 nM in most cases) in the presence or absence of 10 –3 M L-isoproterenol, in a total volume of 450 ... 1978; 10:363–85 ... 1974; 35:316–24 ...