FEBS letters

Rat CYP2D2, not 2D1, is functionally conserved with human CYP2D6 in endogenous morphine formation

N Grobe, TM Kutchan, MH Zenk

文献索引：Grobe, Nadja; Kutchan, Toni M.; Zenk, Meinhart H. FEBS Letters, 2012 , vol. 586, # 13 p. 1749 - 1753

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被引用次数: 5

摘要

Abstract The assumption that CYP2D1 is the corresponding rat cytochrome to human CYP2D6 has been revisited using recombinant proteins in direct enzyme assays. CYP2D1 and 2D2 were incubated with known CYP2D6 substrates, the three morphine precursors thebaine, codeine and (R)-reticuline. Mass spectrometric analysis showed that rat CYP2D2, not 2D1, catalyzed the 3-O-demethylation reaction of thebaine and codeine. In addition, ...