New ratiometric fluorescent calcium indicators with moderately attenuated binding affinities

…, LA Lapham, ME Leonard, ZL Zhou, J Bingham…

文献索引：Gee, Kyle R.; Archer, Eric A.; Lapham, Laura A.; Leonard, Mary E.; Zhou, Zhang-Lin; Bingham, John; Diwu, Zhenjun Bioorganic and Medicinal Chemistry Letters, 2000 , vol. 10, # 14 p. 1515 - 1518

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被引用次数: 31

摘要

Mono-halogenated derivatives of the calcium indicators fura-2 and indo-1 were synthesized and their spectroscopic properties evaluated. Halogenation ortho or para to the bridging oxygen in the BAPTA nucleus had a more pronounced weakening effect on binding affinity than in the meta position in the fura derivatives. Two new excitation ratioable fluorescent calcium indicators, benzothiaza-1 and 2, were also synthesized. Kd values of 400 nM to ...