Chemistry & biology

Enzymatic extender unit generation for in vitro polyketide synthase reactions: structural and func-tional showcasing of Streptomyces coelicolor MatB

AJ Hughes, A Keatinge-Clay

文献索引：Hughes, Amanda J.; Keatinge-Clay, Adrian Chemistry and Biology, 2011 , vol. 18, # 2 p. 165 - 176

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被引用次数: 62

摘要

In vitro experiments with modular polyketide synthases (PKSs) are often limited by the availability of polyketide extender units. To determine the polyketide extender units that can be biocatalytically accessed via promiscuous malonyl-CoA ligases, structural and functional studies were conducted on Streptomyces coelicolor MatB. We demonstrate that this adenylate-forming enzyme is capable of producing most CoA-linked polyketide extender ...